In conclusion, by applying the MPS diagnostic panel targeting 80 deafness genes to 12 multiplex Han Chinese families with idiopathic nonsyndromic SNHI, we identified 4 variants in 4 different genes, which might have led to SNHI in 4 families compatible with autosomal dominant inheritance, including GJB2 p.R75Q, MYO7A p.T381M, KCNQ4 p.S680F, and MYH9 p.E1256K. The gene discussed is MYH9; the disease is deafness.