After OGD 4h followed by re-oxygenation, we observed a significant increase of both TUNEL-positive staining and NDRG2 expression in a time-dependent manner, parallel to the finding in previous in vivo study [7], indicating that this OGD model in IL-6-differentiated C6 glioma cells could mimic the I/R injury induced by transient focal cerebral ischemia in rats. This evidence concerns the gene IL6 and central nervous system cancer.