Similarly, to validate the specific immunoreactivity of anti-AKAP4 antibody with endogenous AKAP4 protein in cancer specimens, polyclonal anti-AKAP4 antibody was preincubated with 15 μg/ml of recombinant AKAP4 protein and used on serial tissue sections of IDC which resulted in complete loss of immunoreactivity (Figure 7F). Here, AKAP4 is linked to cancer.