KCNH1 and cancer: We observed a significant reduction of proliferation in MG-63 cells treated by 20 μM imipramine for 2 days, which is consistent with previous reports.4,28,29 However, the side effects of imipramine limit its applicability in the cancer treatment.6 Compared with imipramine, siRNA is a more specific tool to investigate the role of Eag in cancer progression.