In addition, in accordance with previous findings (Hoffmann et al., 2002; Lin and Karin, 2003; Basak et al., 2012), it was shown that NFκB activation in HCC cell lines only requires TNFα stimulation, and that activation of PI3K signaling in response to insulin is predominantly observed in HCC cell lines. Here, NFKB1 is linked to hepatocellular carcinoma.