Among the pregnant women of the study only one (0.47%) was IgG negative and IgM positive and 8 (3.8%) were both IgG and IgM positive, which means 4.2% of the women had detectable IgM antibodies during pregnancy with potential risk of congenital toxoplasmosis warranting attention to design preventive measures. This evidence concerns the gene CD40LG and congenital toxoplasmosis.