Here, we intended to identify suitable MREs for bladder cancer specificadenovirus-mediated TRAIL expression from the miRNAs with downregulated expressionin bladder cancer, including miR-1 [18-21], miR-99a [22], miR-100 [23], miR-101 [24,25], miR-125b [23,26,27], miR-133a [18,20,21,23,28-30], miR-143 [22,23,31-33], miR-145 [21,23,29-31,34], miR-195-5p [35], miR-199a-3p [36], miR-200 [37,38], miR-203 [39,40], miR-205 [37], miR-218 [21,41], miR-490-5p [42], miR-493 [43], miR-517a [44], miR-574-3p [45], miR-1826 [46] and let-7c [42]. The gene discussed is TNFSF10; the disease is urinary bladder carcinoma.