Obviously, this assay is also more convenient and accurate than the commonly used methods such as traditional western blot and real-time PCR on other invasion parameters including cell-cell adhesion and interaction (E-cadherin and N-CAM loss), cell-matrix (ECM) adhesion and interaction, neoangiogenesis and lymphangiogenesis and so on for tumor invasion investigation [44]–[48]. The gene discussed is NCAM1; the disease is neoplasm.