Similar to our results, two reports on TGF-β-induced EMT in human keratinocytes [24] and a colon carcinoma cell line [27] both identified miR-21 as the major up-regulated miRNA during EMT, and a recent study on TGF-β-induced cardiac EndMT showed that miR-21 promoted EndMT through targeting of the PTEN/Akt pathway [29]. This evidence concerns the gene AKT1 and colon carcinoma.