SPP1 and lung cancer: To further unveil whether osteopontin, LAMB3 and ITGB1 are involved in the metastasis of lung cancer in vivo,4 to 6-week-old female nude mice were randomnly divided into 4 groups and received tail vein injection of 2×106 SPC-A-1sci cells transfected with the shRNA-negative control, shRNA-osteopontin, shRNA-LAMB3 or shRNA-ITGB1.