In the present study, we silenced the FANCF gene by RNA interference in OVCAR3 ovarian cancer cells and found decreased expression of FANCF protein, ratio of FANCD2-L/FANCD2-S and FANCD2 foci, which suggests inactivation of the FA/BRCA pathway by FANCF silencing in OVCAR3 ovarian cancer cells. The gene discussed is FANCF; the disease is ovarian carcinoma.