TRIM63 and Insulin resistance: To determine if PMI5011 regulates Atrogin-1 and MuRF-1 expression in a different in vitro model of skeletal muscle insulin resistance, we assayed the effect of PMI5011 on Atrogin-1 and MuRF-1 expression in the presence of palmitic acid, a fatty acid that inhibits insulin signaling in C2C12 myotubes [30], modeling fatty acid induced insulin resistance.