PROM1 and hepatocellular carcinoma: Because HCC Huh-7 cells have higher percentage of CD133 positive cells compared with other HCC cell lines, such as LM3 and HepG2 cells, we thus silenced CD133 in CD133hi Huh-7 cells by shCD133 and meanwhile isolating CD133+ cells and CD133− cells from Huh-7 cells.