To elucidate the regulatory effect of hGAG on these known signaling pathways in B16F10 tumor cells, the activation of AKT, Jak2, Stat3, FAK, Smad2 and MAPK proteins (p38, p-p38, ERK, p-ERK, JNK and p-JNK) were assessed in the cells treated with hGAG for 24 h/37°C at the indicated concentrations. Here, SMAD2 is linked to neoplasm.