Similarly, considering that PMA could induce activation of protein kinase C (PKC)/mitogen-activated protein kinase kinase (MEK)/ERK pathway [50], [51], the B16F10 tumor cells were treated with PMA at 10 μM alone or in combination with hGAG at the indicated concentration for 24 h/37°C and the TF expression was analyzed. This evidence concerns the gene TF and neoplasm.