In addition, Foxp3+ Treg cells including single positive CD4+Foxp3+ T cells (Figure 3e, p<0.01), and double positive CD4+CD8+Foxp3+ T cells (Figure3f, p<0.001) significantly decreased in the ileum of rats with NEC, compared to normal dam-fed rats. The gene discussed is CD4; the disease is necrotizing enterocolitis.