We found increased Bmi1 expression in MiaPaCa-2 cells did correlate with increased expression of the EMT markers vimentin and ZEB1, while knockdown of Bmi1 in Panc-1 cells lead to downregulation of these markers and Snail (Figure 3B), demonstrating a clear relationship between Bmi1 and expression of EMT markers in pancreatic cancer cells. This evidence concerns the gene VIM and familial pancreatic carcinoma.