Five hypermethylated loci harbored by four genes (GSTP1, TACSTD2, BMP4 and RASSF1) and three hypomethylated loci in three genes (ARHGAP8, GPR35 and DLGAP1) were validated using 7 assays with 12 tumor-normal tissue pairs from this study. This evidence concerns the gene TACSTD2 and neoplasm.