Of the 16 tumor-normal pairs with presumed hypermethylated promoter loci in HCC (in genes BMP4, RASSF1,GSTP1, and TACSTD2), 5, 6, 14 and 16 of the 16 HCCs had decreased expression of the gene, respectively. The gene discussed is RASSF1; the disease is hepatocellular carcinoma.