Based on Western blotting results, the parental HCC cells, including Hep3B, HepG2, PLC/PRF5, SMMC-7721, BEL-7402, SK-Hep-1 and BEL-7404, were all positive for BIRC5, although the expression levels were lower in SMMC-7721, BEL-7402 and BEL-7404 cells than in Hep3B, HepG2, PLC/PRF5 and SK-Hep-1 cells. The gene discussed is BIRC5; the disease is hepatocellular carcinoma.