Univariate analysis of prognostic factors for disease-free survival following hepatic resection in patients with large HCC identified AFP ≥ 1000 ng/mL, decreased serum albumin levels, increased ALP (normal range, 53 to 128 IU/mL) levels, and intrahepatic metastases as statistically significant factors determining poor prognosis (Table 3). This evidence concerns the gene ALB and hepatocellular carcinoma.