(2) We recommend that adult patients after mild head injury with GCS 14 and no risk factors (anticoagulant therapy or coagulation disorders, post-traumatic seizures, clinical signs of depressed or basal skull fracture, focal neurological deficits), or GCS 15 with loss of consciousness or repeated (≥2) vomiting and no other risk factors, be sampled for analysis of S100B if less than 6 h have elapsed following trauma. This evidence concerns the gene S100B and bone fracture.