KL and chronic kidney disease: In the assessment of vascular calcification conducted in the current study, the levels of serum Klotho were decreased in CKD patients with ACI>0% compared to those in patients without aortic calcification (Figure 2D), although the levels of serum Klotho were not significantly correlated with the degree of ACI (Figure S2H) or were not independent determinants of ACI (Table S3).