The AMELY upstream regulation molecular network model was constructed, including BUB1B, CST6, ESM1, HOXA5, LEF1, MAPT, MYBL2, NOTCH3, PLA2G1B, PROK1, ROBO1, SCML2 and UBE2C, in HCC from a GEO dataset by gene regulation network inference methods and computational programming. The gene discussed is PLA2G1B; the disease is hepatocellular carcinoma.