The AMELY-activated upstream regulation molecular network included BUB1B, CST6, ESM1, HOXA5, LEF1, MAPT, MYBL2, NOTCH3, PLA2G1B, PROK1, ROBO1, SCML2 and UBE2C in HCC, as shown in Fig. 2. This evidence concerns the gene CST6 and hepatocellular carcinoma.