The AMELY-activated upstream regulation molecular network included BUB1B, CST6, ESM1, HOXA5, LEF1, MAPT, MYBL2, NOTCH3, PLA2G1B, PROK1, ROBO1, SCML2 and UBE2C in HCC, as shown in Fig. 2. The gene discussed is AMELY; the disease is hepatocellular carcinoma.