We further constructed a model of the AMELY upstream regulation molecular network in HCC only, which included BUB1B, CST6, ESM1, HOXA5, LEF1, MAPT, MYBL2, NOTCH3, PLA2G1B, PROK1, ROBO1, SCML2 and UBE2C, by comparison with the corresponding activated GO molecular network of non-tumor hepatitis/cirrhotic tissues (Fig. 3). The gene discussed is NOTCH3; the disease is hepatocellular carcinoma.