The aim of this study was to compare the biological processes and occurrence numbers of gene ontology (GO) in non-tumor hepatitis/cirrhotic tissues (HBV or HCV infection) with low expression of AMELY upstream regulation networks and the corresponding HCC tissue with high expression (fold change ≥2) of AMELY. AMELY-activated upstream regulation molecular networks in non-tumor hepatitis/cirrhotic tissues and HCC were constructed. The gene discussed is AMELY; the disease is hepatocellular carcinoma.