To determine whether the tumor-induced activation of TLR2/6 enhanced bioactivity of Cyp27B1, thus leading to hCAP18/LL-37 expression, macrophages were exposed to the TLR2-TLR6 ligand Pam2CSK4, in the presence and absence of 25D3 media (DMEM without serum). This evidence concerns the gene TLR6 and neoplasm.