As expected, the numbers of MDSCs in the spleens increased from about 4.3 % in the Tgfbr1f/f/Ptenf/f mice to 32% in the untreated Tgfbr1/Pten 2cKO mice due to deletion of TGFβRI and PTEN and to the subsequent tumor development. Here, PTEN is linked to neoplasm.