FOXO3 and hepatocellular carcinoma: The cell count kit-8 assay demonstrated that cells transfected with negative control siRNA initially proliferated in the absence of 10% FBS; however, FOXO3a-siRNA interference reduced the growth curve and accelerated a decline in the viability of HCC cells in the absence of 10% FBS (Figure 3B (a); SFM + FOXO3a-siRNA vs. SFM + NC-siRNA, **P < 0.01 for Huh-7 and Hep3B, two-way ANOVA; SFM + FOXO3a-siRNA vs. SFM + NC-siRNA, **P<0.01 for HepG2, t-test).