To correlate our in vitro findings of increased acinar cell production of IL-6, CXCL2/MIP-2α and CCL2/MCP-1 in response to IL-33 stimulation with their expression in vivo in our experimental model, we assayed these cytokines in the pancreas and lung in ligation-induced acute pancreatitis in mice using ELISA. This evidence concerns the gene IL33 and acute pancreatitis.