To interrogate which PRR is essential for mycobacteria-induced MCP-2/CCL8 production in macrophages, we compared mRNA levels of mcp-2/ccl8 among macrophages from wild-type, TLR2−/−, TLR3−/− or TLR4−/− mice after infection with either BCG or H37Rv at an MOI 5 for 24 h. The gene discussed is TLR4; the disease is infection.