Analysis using the Aldefluor assay kit on H1650 and H1975 cell lines showed 8 and 6 fold higher frequency of Aldh-Hi cells in SP cells as compared to MP cells, suggesting that SP cells isolated from NSCLC cell lines are enriched in stem like cells (Figure 1D), as seen by the expression of a different marker. This evidence concerns the gene LDHA and non-small cell lung carcinoma.