Anyhow, since some ANO1+/SMCα-actin negative cells are also Wt1cre-YFP, and also given the YFP/c-Kit immunocolocalization observed in presumptive ICC and the expression of c-Kit detected by RT-PCR in purified Wt1cre-YFP cells, we can conclude that cells from the Wt1 lineage are involved at least in the earliest stages of ICC differentiation. This evidence concerns the gene WT1 and intrahepatic cholangiocarcinoma.