Anyhow, since some ANO1+/SMCα-actin negative cells are also Wt1cre-YFP, and also given the YFP/c-Kit immunocolocalization observed in presumptive ICC and the expression of c-Kit detected by RT-PCR in purified Wt1cre-YFP cells, we can conclude that cells from the Wt1 lineage are involved at least in the earliest stages of ICC differentiation. The gene discussed is ANO1; the disease is intrahepatic cholangiocarcinoma.