With multivariate analysis using a Cox regression model, Child-Pugh class (p < 0.001; Hazard ratio [HR] 1.711; 95% confidence interval [CI] 1.377–2.125), tumor size > 5 cm (p = 0.003; HR 1.778; 95% CI 1.209–2.615), tumor multiplicity (p = 0.035; HR 1.391; 95% CI 1.023–1.892), presence of PVT (p = 0.001; HR 1.827; 95% CI 1.284–2.598), AFP > 200 ng/mL (p = 0.001; HR 1.734; 95% CI 1.248–2.407), CRP > 6.3 mg/L (p = 0.027; HR 1.519; 95% CI 1.049–2.199) and NLR > 2.3 (p = 0.009; HR 1.601; 95% CI 1.124–2.280) were identified as independent poor prognostic factors for HCC (Table 2). The gene discussed is AFP; the disease is hepatocellular carcinoma.