CD28 and type 1 diabetes mellitus: Results from two representative subjects of either T1D patient or healthy controls are shown in Fig. 8A–B. Following a 3-day incubation of autologous PBLs with expanded Treg plus anti-CD3/CD28, the proliferation of CFSE-labeled CD8+ Teff cells in PBLs was analyzed at the end of the assay.