In contrast, following infection with wild-type S. Typhi for 4 h (Figure 6B; MOI 400:1), ZO-1 distribution appears altered: while still observing the protein at the cell boundaries, ZO-1 also appeared clustered into the cytoplasm and co-localized with aggregates of actin fibers of the disrupted cytoskeleton (Figures 6D,F). The gene discussed is TJP1; the disease is infection.