TP53 and neoplasm: Using this antibody, we observed DNA damage-induced phosphorylation of endogenous Daxx in a range of other tumor or primary cell lines including the p53-wild-type HT1080 and IMR90 cells and the p53-deficient H1299, Saos-2, HeLa, and 293T cells (Figure 2B), indicating its generality and its independence of p53 status.