As the experimental model of AE allowed us to study the correlation, if any, between T lymphocyte infiltration in the liver and TGF-β expression over the time course of infection, CD4 and CD8 immunostaining was performed in the liver of mice; this was not performed in the liver of AE patients, since the time course could not be assessed. This evidence concerns the gene CD4 and acrodermatitis enteropathica.