In this study, we have investigated and compared the effects of IL-2, IL-15 and IL-21 cytokine on the expansion, phenotype and function of TIL from NSCLC, OvCa or melanoma biopsies utilizing aAPC engineered to express the Fcγ receptor CD32 (for anti-CD3 mAb binding), the co-stimulatory molecule 4-1BBL and either IL-2, IL-15 or IL-21. Here, IL2 is linked to melanoma.