The evaluation of the percentage of apoptotic cells by cytofluorimetric analysis of propidium iodide incorporation and Annexin V binding revealed an increase of ∼15% in cell death in treated cells with respect to cells either untreated or treated with an unrelated antagomiR (i.e., the antisense sequence targeting miR-133a, whose expression is totally absent in melanoma, our unpublished result) (Fig. 5g, left). This evidence concerns the gene ANXA5 and melanoma.