To understand the clinical significance of miR-130a/301a/454 and its target in colon cancer, we determined the levels of miR-130a/301a/454 and protein expression of Smad4 in 14 pairs of matched colon cancer specimens by qRT-PCR and immunoblotting, respectively (Fig. 5A and B). Here, SMAD4 is linked to malignant colon neoplasm.