Among the genes with NSph-T-DMRs, we identified human gene orthologs involved in neurological diseases, such as spinocerebellar ataxia type 1 (ATXN1 and KAT5), schizophrenia (BLOC1S1, NTNG1, and OLIG2), autism (NRCAM), and brain malformation syndrome (LIG4 and NFIA) (Additional file2: Table S1). The gene discussed is LIG4; the disease is nervous system disorder.