AKT1 and hepatocellular carcinoma: As shown in Fig. 2A and B, bortezomib downregulated the protein levels of CIP2A, P-Akt, P-4EBP1 and induced autophagy in all HCC cell lines, including Huh-7, Sk-Hep1 and Hep3B, in a concentration- and time- dependent manner.