To provide direct evidence that FoxQ1 affect the malignant phenotype by down-regulation of NRXN3 in glioma cells, we transfected NRXN3 shRNA into FoxQ1-shRNA2 to rescue the NRXN3 expression and established the stable clone (NRXN3-rescue). This evidence concerns the gene NRXN3 and central nervous system cancer.