To assess whether TMZ induces autophagy in glioma cells, LN-229 (a glioblastoma line) and U87 MG (an astrocytoma line) were treated with 100 μM TMZ and stained with monodansylcadaverine (MDC) 24–144 h after TMZ exposure (cells were pretreated with O6BG in order to deplete residual MGMT activity). Here, MGMT is linked to astrocytoma (excluding glioblastoma).