On infection with Ad:EV, Ad:IGFBP-3 and Ad:IGFBP-3GGG mutant, semi-quantitative PCR shows that the effect of Ad:IGFBP-3GGG on the mRNA levels of GLUT4, IRS-1, adiponectin and MCP-1 was similar to Ad:IGFBP-3 suggesting that IGFBP-3 effect on the NF-κB pathway in adipocytes was IGF-independent (Figure 3C). This evidence concerns the gene IRS1 and infection.