CCNE1 and central nervous system cancer: Moreover, as shown in Supplemental Figure 4, the levels of Cyclin D1 and Cyclin E1 proteins were upregulated in glioma cell lines relative to normal astrocytes, and in tumors relative to adjacent normal tissue, which further confirm the notion that downregulation of miR-195 could lead to upregulation of Cyclin D1 and Cyclin E1 expression in glioma.