We also showed that mTOR inhibitors inhibited the proliferation of CD34+ cells and hematopoietic colony formation from MPN patients at doses significantly lower than healthy subjects and potently reduced the generation of erythroid-independent colonies (EEC) that are considered to closely represent the MPN clone since they are mostly JAK2V617F mutated [10], [49]. This evidence concerns the gene CD34 and myeloproliferative disorder.