A multivariate survival analysis using Cox’s proportional hazard model for the selection of the prognostic factors for disease-free survival, including clinicopathological parameters mentioned above and immunofluorescence costaining results of the blood vessel density and nucleolin expression level, indicated that both nucleolin expression on tumor vessels and clinical stage III are independent factors of poor prognosis for disease-free survival (P = 0.003 and P<0.0001, respectively,Table 3). The gene discussed is NUCLEOLIN; the disease is neoplasm.