In this study, we verified SATB1 was overexpressed in the SCLC tissues and metastatic lymph nodes tissues, small interfering RNA targeting SATB1- SATB1- siRNA-1 and SATB1-siRNA-2 were constructed successfully, our results showed that transfection with SATB1- siRNA-1 or SATB1-siRNA-2 into the SCLC cell-NCI-H446, could inhibit the cells proliferation and invasion significantly. The gene discussed is SATB1; the disease is small cell lung carcinoma.