The function and phenotype of Treg cells and the expression of PD-1 and PD-L1 on different cell populations from the peripheral blood of patients with high-risk-resected stage III and IV melanoma has been studied, and PD-1 blockage found to augment the generation of melanoma antigen-specific cytotoxic T cells by stimulating their proliferation and, indirectly, by masking their suppression by Treg cells (Wang et al., 2009). This evidence concerns the gene CD274 and melanoma.