In a syngeneic murine glioma model, combining Treg cell depletion with administration of blocking CTLA-4 mAbs further boosted glioma-specific CD4+ and CD8+ effector T cells resulting in complete tumor eradication without any signs of autoimmunity. These data illustrate that intratumoral accumulation and activation of CD4+FoxP3+ Treg cells act as a dominant immune escape mechanism for gliomas. The gene discussed is CD4; the disease is central nervous system cancer.