Previous studies on co-localization between LGR-5 and nuclear β-catenin, performed in EGFP-creERT2/Apc flox/flox mice, showed that cells expressing both LGR-5 (chimeric) and intense nuclear β-catenin staining give rise to daughter cells which still accumulate nuclear β-catenin but lose the EGFP-LGR-5 positiveness, suggesting that the simultaneous expression of both markers identify cancer stem cells [30]. Here, APC is linked to cancer.