In this study, bladder cancers showed upregulated expression of 14-3-3σ (spot 2) whereas myosin regulatory light chain 2 (spot 1), galectin-1 (spot 3), lipid-binding AI (spot 4), annexin V (spot 5), transthyretin (spot 6), CARD-inhibitor of NF-κB-activating ligand (spot 8) and actin prepeptide (spot 9) were downregulated. The gene discussed is LGALS1; the disease is urinary bladder carcinoma.