Further canonical pathways whose significance increased with cancer progression were cytoskeleton and motility/invasion (‘Actin cytoskeleton signaling’, ‘Regulation of actin-based motility by Rho’ and ‘Ephrin signaling’) (Table S1); adhesion (‘Integrin signaling’, ‘ILK signaling’ and ‘FAK signaling’) (Table S2); and stromal response, with higher expression and number of ECM genes seen as PanINs progress to PDAC (Figure 4B). This evidence concerns the gene RHO and cancer.